DEPARTMENT OF VETERINARY SCIENCE
Thursdays
2:30-4:25 Room 9 Henning Building 2 Credits
Instructor: Robert Paulson Office 104 Henning
Phone 863-6306
email
rfp5@psu.edu
Format:
During each class we will analyze two to three papers from the primary
literature. Students will present the papers and the class will discuss the
each paper. The emphasis of the course will be on understanding the techniques
used and how the results of the paper fit into the broader context of
Developmental biology.
Grading:
Each student will be graded on their presentations and their participation in
class.
Syllabus:
|
August |
23 |
Introduction
to hematopoiesis, mouse, frog and fish development. (Bob Paulson). |
|
|
30 |
Mesoderm
formation in mice: Role of the Ectoderm in patterning The mesoderm. |
|
September |
6 |
Patterning
the mesoderm in Frogs: Factors that induce mesoderm and Fate mapping
hematopoietic tissue. |
|
|
13 |
Origins
of hematopoietic and endothelial cells in Zebrafish: a genetic approach. |
|
|
20 |
Hemangioblasts-bipotential
progenitors of hematopoietic and endothelial cells. |
|
|
27 |
Flk-1
and Sci markers for hemangioblast. |
|
October |
4 |
Using ES
cells to study hemangioblast development I I The origin of Primitive and definitive
progenitors |
|
|
18 |
The origin of hematopoietic stem cells in the embryo |
|
25 |
The
role of the yolk sac in definitive hematopoiesis |
|
November |
1 |
Runx1
and the development of definitive hematopoiesis in the fetal
liver |
|
|
8 |
Adhesion
molecules and the homing of stem cells to the fetal liver and bone marrow |
|
|
15 |
Role of receptor tyrosine kinases in vasculogenesis |
|
|
22 |
Thanksgiving |
|
|
29 |
Angiogenesis
and the remodeling of the vasculature.
|
|
December |
6 |
The role of oxygen in the development of the hematopoietic and vascular system. |
August 23
Introduction
to hematopoiesis, mouse, frog and fish development.(Bob Paulson)
August 30
Mesoderm
formation in mice- Role of the Ectoderm in patterning the mesoderm.
Hematopoictic
induction and respecification of A-P identity by visceral endoderm signaling in
the mouse embryo. M. Belaoussoff et al. Development 125:5009-5019 (1998).
Indian
hedgehog activates hematopoiesis and vasculogenesis and can respecify
prospective neurectodermal cell fate in the mouse embryo. Dyer MA, Farrington
SM, Mohn D, Munday M Baron ME. Development. (2001) 128:1717-1730.
September 6
Patterning
the mesoderm in Frogs: Factors that induce mesoderm and Fate mapping
hematopoietic tissue.
The
origins of primitive blood in Xenopus: implications for axial patterning. MC
Lane and WC Smith (1999) Development 128:423-434.
Bipotential
primitive-definitive hematopoietic progenitors in the vertebrate embryo. J
Turpen, C Kelley, P Mead and L Zon. Immunity (1997) 7:325-334.
The role
of BNV-4 and GATA-2 in the induction and differentiation of hematopoietic
mesoderm in Xenopus laevis. Maeno M, Mead PE, Kelley C, Xu RH, Kung HF, Suzuki
A, Ueno N, Zon LI. Blood. (1996) 88:1965-1972.
September 13
Origin of hematopoietic cells and endothelial cells in Zebrafish.
Cloche,
an early acting zebrafish gene, is required by both the endothelial and
hematopoietic lineages. D Stanier et al. Development (1995) 121:3141-3150.
Cell-autonomous
and non-autonomous requirements for the zebraffsh gene cloche in hematopoiesis.
Parker L, Stainier DY. Development. (I 999) 126:2643 -5 1.
The Scl
gene specifies haemangioblast development from early mesoderm. M Gering et al.
EMBO Journal (1998) 17:4029-4045.
September 20
Hemangioblasts-bipotential
progenitors of hematopoietic and endothelial cells: evidence from avian embryos.
Two
distinct endothelial lineages in ontogeny, one of them related to hemopoiesis.
Pardanaud L, Luton D, Prigent M, Bourcheix LM, Catala M, Dieterlen-Lievre F.
Development. (1996) 122:1363-1371.
Manipulation
of the angiopoietic/hemangiopoietic commitment in the avian embryo, Pardanaud L,
Dieterlen-Lievre F. Development. (1999) 126:617-627.
Tracing
the progeny of the aortic hemangioblast in the avian embryo. T Jaffredo et al.
Developmental Biology (2000) 224:204-214.
September 27
Flk-I and
Sel: markers for the hemangioblast.
Failureofblood-islandformationandvasculogenesisinFIk-l-deficientmice. ShalabyF,
Rossant J, Yamaguchi TP, Gertsenstein M, Wu X:F, Breitman ML, Schuh AC. Nature.
(1995) 376:62-66.
A
requirement for Flkl in primitive and definitive hematopoiesis and
vasculogenesis Shalaby F, Ho J, Stanford WL, Fischer KD, Schuh AC, Schwartz L,
Bernstein A, Rossant J. Cell. (1997) 89:981-90.
SCL
specifies hematopoietic mesoderm in Xenopus embryos. Development. Mead PE,
Kelley CM, Hahn PS, Piedad 0, Zon LI. (1998) 125:2611-2620.
Unsuspected
role for the T-cell leukemia protein SCL/tal- I in vascular development.
Visvader JE, Fujiwara Y, Orkin SH. Genes Dev. 1998 Feb 15; 12(4):473 -9.
October 4
Using ES cells to study hemangioblast development
A
Common Precursor for hematopoietic and endothelial cells. K Choi et al.
Developemnt (1998) 125:725-732.
Basic
fibroblast growth factor positively regulates hematopoietic development. P
Faloon et al. Development (2000) 127:1931-1941.
A
transitional stage in the commitment of mesoderm to hematopoiesis requiring the
transcription factor Scl/tal-1. SM Robertson et al. Development (2000)
127:2447-2459.
Regulation
of hemangioblast development. Lacaud G. et al. Annal of the NY Academy of
Science (2001) 938:96-107. Review
October 11
The origin of Primitive and
definitive progenitors
Properties
of the earliest clonogenic hemopoietic precursors to appear in the developing
murine yolk sac. Wong PM, Chung SW, Chui DH, Eaves CJ. Proc Nati Acad Sci U S
A. (1986) 83:3851-3854.
Development
of erythroid and myeloid progenitors in the yolk sac and embryo proper of the
mouse. Palis J, Robertson S, Kennedy M, Wall C, Keller G Development. (1999)
126:5073-5084.
In
vitro development of primitive and definitive erythrocytes from different
precursors. T Nakano, et al. Science (1996) 272:722-724.
A
common precursor for primitive erythropoiesis and definitive haematopoiesis.
Kennedy M, Firpo M, Choi K, Wall C, Robertson S, Kabrun N, Keller G. Nature. (I 997) 386:488-493.
Yolk-sac
hematopoicsis. The first blood cells of mouse and man. Palis J, Yoder MC. Exp
Hematol. (2001) 29:927-936. Review
October 18
The origin of hematopoietic stem
cells in the embryo
An
early pre-liver intraembryonic source of CFU-S in the developing mouse.
Medvinsky AL, Samoylina NL, Muller AM, Dzierzak EA Nature. (I 993) 3 64:64-67.
Definitive
hematopoiesis is autonomously initiated by the AGM region. Medvinsky A,
Dzierzak E. Cell. (I 996) 86:897-906.
Characterization
of the first definitive hematopoietic stem cells in the AGM and liver of the
mouse embryo. Sanchez MJ, Holmes A, Miles C, Dzierzak E. Immunity. (1996)
5:513-25.
October 25
The role of the yolk sac in
definitive hematopoiesis
Characterization
of definitive lymphohematopietic stem cells in the day 9 murine yolk sac. M
Yoder, et al. Immunity (1997) 7: 335-344.
Spatial
and temporal emergence of high proliferative potential hematopoietic precursors
during murine embryogenesis. J Palis, et al. PNAS (2001) 98:4528-4533.
Generation
of definitive hematopoietic stem cells from murine early yolk sac and
paraaortic splanchnopleures by aorta-gonad-mesonephros region-derived stromal
cells. Sahoko Matsuoka, et al. Blood (2001) 98: 6-12.
Introduction:
spatial origin of murine hematopoietic stem cells. M Yoder Blood (2001) 98:
3-5. Review
November 1
Runxl and the development of definitive hematopoiesis in the fetal
Liver.
Cbfa2
is required for the formation of intra-aortic hematopoietic clusters. North T,
Gu TL, Stacy T, Wang Q, Howard L, Binder M, Marin-Padilia M, Speck NA
Development. (1999) 126:2563-2575.
Haploinsufficiency
of ANILI affects the temporal and spatial generation of hematopoietic stem
cells in the mouse embryo. Cai Z, de Bruijn M, Ma X, Dortland B, Luteijn T,
Downing RJ, Dzierzak E. Immunity. (2000) 13:423-43 1.
The AUL
I transcription factor functions to develop and maintain hematogenic precursor
cells in the embryonic aorta-gonad-mesonephros region. Y Mukouyama et al.
Developmental Biology (2000) 220:27-36.
Potential
roles for RUNX I and its orthologs in determining hematopoietic cell fate.
Tracey V;]D, Speck NA. Sen@n Cell Dev Biol. (2000) 11:337-342. Review
November 8
Adhesion molecules and the homing of stem cells to the fetal liver and
bone
marrow
Impaired
migration but not differentiation of hematopoietic stem cells in the absence of
DI integrins. E Mrsch, et al. Nature (1996) 380:171-175.
Fetal
and adult hematopoietic stem cells require 0 1 integrin function for colonizing
fetal liver, spleen and bone marrow. A Potocnik, et al. Immunity (2000)
12:653-663.
(x4
integrins regulate the proliferation/differentiation balance of multilineage
hematopoietic progenitors in vivo. A Arroyo, et al. Immunity (1999) 11:555-566.
November
15 Role of receptor tyrosine kinases in vasculogenesis
Flkl
positive cells derived from embryonic stem cells serve as vascular progenitors.
J Yamashita, et al. Nature (2000) 408:92-96.
Interaction
of the TEK and TIE receptor tyrosine kinases during cardiovascular development.
Puri MC, Partanen J, Rossant J, Bernstein A. Development. (I 999) 126:4569-4580
Abnormal
blood vessel development and lethality in embryos lacking a single VF-GF
aliele. Carmeliet P, Ferreira V, Breier G, Pollefeyt S, Kieckens L, Gertsenstein
M, Fahrig M, Vandenhoeck A, Harpal K, Eberhardt C, Declercq C, Pawling J, Moons
L, Collen D, Risau W, Nagy A. Nature. (1996) 380:435-439.
Vasculogenesis
in the mouse day 6.5 to 9.5 embryo. C Drake et al., Blood (2000) 95:1671-1679.
November 22
Thanksgiving
November 29
Angiogenesis and the remodeling of the
vasculature.
Requisite
role of angiopoietin- 1, a ligand for the TIE2 receptor, during embryonic
angiogenesis.
Suri C, Jones PF, Patan S, Bartunkova S, Maisonpierre PC, Davis S, Sato TN,
Yancopoulos GD. Cell. (1996) 87:1171-1180.
Role of
PDGF-B and PDGFR-beta in recruitment of vascular smooth muscle cells and
pericytes during embryonic blood vessel formation in the mouse. Hellstrom M,
Kal n M, Lindahl P, Abramsson A, Betsholtz C. Development. (1999)
126:3047-3055.
December 6
The role of oxygen in the development of the hematopoietic and vascular
system.
A
developmental transition in definitive erythropoiesis: erythropoietin
expression is sequentially regulated by retinoic acid receptors and HNF4.
Takako Makita, Gabriela Hemandez-Hoyos, Tim Hung-Po Chen, Hong Wu, Ellen V.
Rothenberg, and Henry M. Sucov. Genes Dev. (2001) 15: 889-901.
Multilineage
embryonic hematopoiesis requires hypoxic ARNT activity. David M. Adelman, Emin
Maltepe, and M. Celeste Simon Genes Dev. (1999) 13: 2478-2483.